Detection of antibodies to glycosphingolipids using...

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/06 (2006.01) G01N 33/53 (2006.01) G01N 33/531 (2006.01) G01N 33/533 (2006.01) G01N 33/536 (2006.01) G01N 33/537 (2006.01) G01N 33/541 (2006.01) G01N 33/543 (2006.01) G01N 33/551 (2006.01) G01N 33/563 (2006.01) G01N 33/564 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2384278

Methods of detecting antibodies to one or more glycosphingolipd(s) of interest in a sample are disclosed which comprise using a solid-phase reactant having carbonyl groups attached thereon, and the glycosphingolipid(s) of interest linked to the solid-phase reactant by an amide bond between an amino group of the glycosphingolipid of interest and a carbonyl group attached to the solid-phase reactant. The methods of detecting antibodies to glycosphingolipid(s) of interest can be used in methods of diagnosing autoimmune diseases in an individual.

L'invention concerne des procédés de détection d'anticorps dirigés contre un ou plusieurs glycosphingolipide(s) présentant un intérêt dans un échantillon. Ce procédé consiste à utiliser un réactif en phase solide sur lequel sont fixés des groupes carbonyles, et qui contient le(s) glycosphingolipide(s) présentant un intérêt, liés au réactif en phase solide par une liaison amide entre un groupe aminé du glycosphingolipide présentant un intérêt et un groupe carbonyle fixé au réactif en phase solide. Les procédés de détection d'anticorps dirigés contre le(s) glycosphingolipide(s) présentant un intérêt peuvent être utilisés dans des procédés de diagnostic de maladies auto-immunes chez un individu.

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