Detection of antigens and nucleic acids

Details Detection of antigens and nucleic acids Detection of antigens and nucleic acids

G - Physics

01

N

G01N 33/543 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 1/34 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2124585

2124585 9408239 PCTABS00032 A novel application for leucocyte retention filters is provided in the form of a method and kit for isolating and/or identifying foreign antigens and/or nucleic acids associated with leucocytes in fluids derived from a human or animal body, and treating, preferably with detergent, in a manner such as to expose them for isolation and/or detection. The method and apparatus provides a simple to obtain yet rapid determination of foreign antigens and/or nucleic acids associated with the leucocytes and thus the likely presence of organisms of which these antigens are characteristic. One significant advantage of this method is that it offers the preparation of a sample outside the laboratory using a few reagents, a syringe and a filter. Using this method the inventors have assembled a pen-side test that can detect animals persistently infected with BVD virus that takes only 1.5 hours, compared to the 7 days required for a virus isolation test.

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