Detection of apoptotic cells in living nematodes

Details Detection of apoptotic cells in living nematodes Detection of apoptotic cells in living nematodes

G - Physics

01

N

G01N 33/50 (2006.01)

Patent

CA 2335549

The present invention relates to methods and kits for detecting apoptotic cells in a live nematode by exposing the live nematode to a vital dye that stains apoptotic cells, and detecting the apoptotic cells. Applications of this assay include methods of identifying agents, conditions or genes that modulate apoptosis. The present invention also includes methods of screening for mutated nematodes that exhibit altered apoptotic cell death.

La présente invention concerne des méthodes et des kits permettant de détecter des cellules d'apoptose dans un nématode vivant, ces méthodes consistant à exposer ce nématode vivant à un colorant vital conçu pour tacher lesdites cellules d'apoptose, puis à détecter celles-ci. Cette analyse peut notamment être appliquée à des méthodes d'identification d'agents, d'états, ou de gènes qui modulent l'apoptose. La présente invention concerne également des méthodes permettant de détecter par criblage des nématodes mutés présentant une mort cellulaire programmée modifiée.

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