Detection of bacteria by nucleic acid hybridization

Details Detection of bacteria by nucleic acid hybridization Detection of bacteria by nucleic acid hybridization

C - Chemistry, Metallurgy

12

Q

167/37, 150/8.5

C12Q 1/68 (2006.01)

Patent

CA 1231303

ABSTRACT OF THE DISCLOSURE A nucleic acid hybridization method for detecting bacteria in a test sample, such as a mammalian body fluid, employing a polynucleotide probe having a base sequence which is homologous with at least one base sequence in the nucleic acid content or substantially all of the respective bacteria suspected to be present in the sample. The homologous base sequence in the probe preferably comprises at least a 10 base portion of one of the strands of a gene which codes for the synthesis of a nucleic acid or protein comprised in the mechanism of protein synthesis, e.g., tRNAs, rRNAs, and elongation factors. Resulting hybridization between the probe and single stranded nucleic acids released from bacteria in the sample indicates bacterial presence and can be detected by employing labeled probes or otherwise detecting duplex formation. The method pro- vides a unique, direct and rapid means for detecting the presence of bacteria without the need for conventional microbiological culturing. In particular, the method can be applied conveniently as a screening test for bac- terial presence in fluids which are normally sterile, such as urine or blood.

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