Detection of bacteria in fluids

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/04 (2006.01)

Patent

CA 2577260

A method for the detection of bacteria in aqueous fluids in the form of solutions, emulsions and or suspensions, such as drinking water, liquid food and drinks and biological fluids such as urine, spinal and or amniotic fluids and serums by measuring light scattering The fluid suspected of containing bacteria is filtered first to exclude particles of sizes larger than the size of the expected bacteria. Other filtration methods are used in addition to exclude different constituents of the examined fluid. Ion exchange chromatography is such a method. The verification of bacterial contamination and the derivation of its level is performed by matching measured scattering profiles with pre-stored calibrated scattering profiles. A system for carrying out the measurements including cuvette units suited for light scattering measurements is provided.

L'invention concerne un procédé de détection de bactéries dans des fluides aqueux se présentant sous la forme de solutions, d'émulsions et/ou de suspensions, telles que de l'eau potable, des aliments liquides, des boissons et des liquides biologiques tels que l'urine, le liquide spinal et/ou amniotique et des sérums, par la mesure de la diffusion de la lumière. Le fluide suspecté de contenir des bactéries est d'abord filtré de sorte à exclure des particules présentant une taille supérieure à celle des bactéries possibles. D'autres procédés de filtration sont utilisés en plus pour exclure différents constituants du fluide examiné. La chromatographie par échange d'ions est un de ces procédés. La vérification de la contamination bactérienne et la définition d'un niveau de contamination sont effectuées par la mise en correspondance de profils de diffusion mesurés avec des profils de diffusion étalonnés stockés. L'invention concerne également un système permettant d'effectuer des mesures, ledit système comprenant des unités cuvettes adaptées aux mesures de diffusion de la lumière.

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