Detection of bacteria using a nucleic acid amplification

Details Detection of bacteria using a nucleic acid amplification Detection of bacteria using a nucleic acid amplification

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2062078

A b s t r a c t Method for the specific detection of bacteria in a sample by reacting the sample with one or several labelled nucleotide triphosphates and one or several enzymes which catalyze the production of a labelled nucleic acid B containing this nucleotide, thermally denaturing, reacting the sample with a nucleic acid probe C which is sufficiently complementary to nucleic acid B and contains at least one immobilizable group, contacting the nucleic acid hybrid D which may have formed with a solid phase which recognizes and binds the immobilizable group, removing the liquid from the solid phase and determining the label on the solid phase as a measure for the presence of the bacterium.

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