Detection of binding of charged species using ph- or...

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G01N 33/58 (2006.01) C07D 311/16 (2006.01) C12Q 1/68 (2006.01) G01N 33/543 (2006.01) G01N 33/92 (2006.01)

Patent

CA 2399911

The extent of binding of a moiety, such as a biomolecule, to a surface, where the local environment at the surface has a pH or surface potential which is altered upon binding of the moiety, is determined by stably incorporating at the surface a probe which contains a pH- or potential-sensitive fluorophore.

On détermine l'ampleur d'une liaison d'un fragment, tel qu'une biomolécule, à une surface dont l'environnement local présente un pH ou un potentiel qui se modifie lors de la liaison dudit fragment, en incorporant au niveau de la surface une sonde qui contient un fluorophore sensible au pH ou au potentiel.

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