Detection of biological and chemical agents

Details Detection of biological and chemical agents Detection of biological and chemical agents

G - Physics

01

N

G01N 1/00 (2006.01) C12Q 1/70 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2554441

Techniques for the sensitive detection of analytes which combine the benefits of solution/suspension phase assay formats and the simplicity of solid phase/lateral flow assays are described. The assays can be performed in the solution/suspension phase using magnetic microspheres as a solid support. Subsequently a magnetic separation can be performed to separate the bound analyte from the remainder of the solution. After a wash step, the fluorescence signal can be directly read from the magnetic particle surface. Portable biodetection systems which employ fluorescent polymer superquenching and methods for detecting bioagents therewith are also described.

L'invention concerne des techniques de détection sensible d'analytes qui combinent les bénéfices de formats de dosage de phase de solution/suspension et la simplicité de dosages de phase solide/d'écoulement latéral. Lesdits dosages peuvent être réalisés dans la phase de solution/suspension, au moyen de microsphères magnétiques en tant que support solide. Par conséquent, une séparation magnétique peut être effectuée afin de séparer l'analyte lié du reste de la solution. Après une étape de lavage, le signal de fluorescence peut être lu directement de la surface particulaire magnétique. Cette invention a aussi trait à des systèmes de biodétection portables qui utilisent une désactivation amplifiée polymère fluorescente, et à des méthodes de détection de bioagents.

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