Detection of biological molecules by differential...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/00 (2006.01) C12Q 1/04 (2006.01) G01N 21/64 (2006.01)

Patent

CA 2497555

This invention relates to a method and apparatus for detecting a biological molecule associated with enzyme activity in a sample. The invention is applicable to detecting a microorganism associated with an enzyme in a sample such as water, food, soil, or a biological sample. According to a preferred embodiment of the method of the invention, a sample containing an enzyme of interest or a microorganism associated with the enzyme is combined with a suitable substrate, and a fluorescent product of the enzyme-substrate reaction is selectively detected. The fluorescent product is detected with a partitioning element or optical probe/partitioning element of the invention. In one embodiment the partitioning element provides for partitioning of only the fluorescent product molecule into the probe. The invention also provides an automated system for monitoring for biological contamination of water or other samples.

L'invention concerne un procédé et un appareil de détection d'une molécule biologique associée à une activité enzymatique dans un échantillon. Cette invention peut servir à la détection d'un micro-organisme associé à une enzyme dans un échantillon tel de l'eau, de la nourriture, de la terre, ou un échantillon biologique. Selon un mode de réalisation préféré de l'invention, un échantillon contenant une enzyme d'intérêt ou un micro-organisme associé à l'enzyme est combiné avec un substrat convenable, et un produit fluorescent de la réaction enzyme-substrat est sélectivement détecté. Le produit fluorescent est détecté au moyen d'un élément de division ou un élément de sonde/division optique de l'invention. Dans un mode de réalisation, l'élément permet uniquement la division de la molécule du produit fluorescent dans la sonde. Cette invention concerne aussi un système automatique de surveillance de pollution biologique de l'eau ou d'autres échantillons.

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