Detection of biomolecules

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12N 15/00 (2006.01)

Patent

CA 2213622

The present invention concerns a method for detecting the presence of a catalytically active ribozyme in a medium. The detection of the catalytically active ribozyme may be a goal by itself, or the ribozyme may serve as a reporter for the presence of other biomolecules in an assayed sample. The detection is carried out in a catalytic system wherein the presence of the active ribozyme serves to produce other active ribozyme in a positive-feedback amplificatory manner.

L'invention concerne un procédé qui permet de détecter la présence de ribozymes à activité catalytique dans un milieu. Détecter ces ribozymes à activité catalytique peut être un objectif en soi, mais le ribozyme peut aussi servir de rapporteur afin d'indiquer la présence d'autres biomolécules à l'intérieur d'un échantillon analysé. La détection s'effectue dans un système catalytique dans lequel la présence du ribozyme actif sert à produire d'autres ribozymes actifs à la manière d'une amplification à rétroaction directe.

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