G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 30/36 (2006.01) G01N 30/72 (2006.01) G01N 33/15 (2006.01) G01N 33/49 (2006.01)
Patent
CA 2685357
A detection method of blood plasma schizadrin B of dissipating blood stasis botanical is disclosed. The method includes: (1) extracting schizadrin B from plasma of mammalian administered dissipating blood stasis botanical by ethyl acetate with the volume ratio 1:4, whirling 3-5mins, centrifugating at 9600rpm for 10mins, drying and enriching the upper layer at 25-30°C, and redissolving with mobile phase; (2) UPLC/MS measuring: UPLC condition: chromatographic column: Acquity UPLC BEH C18, 2.1´100mm, mobile phase A: water-acetonitrile-formic acid 95:5:0.1 v/v/v, mobile phase B: acetonitrile-formic acid 100:0.1 v/v; MS condition: electric spraying ion source(ESI), detecting with positive ion mode, scanning at the range of m/z 150-1200. The method can be used for pharmacokinetics study of schizadrin B in dissipating blood stasis botanical.
Procédé de détection de schizadrine B dans le plasma sanguin après administration de médicament botanique dissipant la stase sanguine, qui comprend les étapes suivantes; (1) extraction de la schizadrine B depuis le plasma d'un mammifère auquel on a administré le produit dissipant la stase sanguine, au moyen d'acétate d'éthyle, selon un rapport volumique de 1:4, brassage sur une durée comprise entre 3 et 5 minutes, centrifugation à 9600 tours/minute pendant 10minutes, séchage et enrichissement de la couche supérieure pendant une durée comprise entre 25 et 30°C et redissolution en phase mobile; (2) mesure UPLC/MS: état UPLC: colonne de chromatographie: Acquity UPLC BEH C18, 2.1´100mm, phase mobile A: eau-acétonitrile-acide formique: 95:5:0.1 v/v/v, phase mobile B: acétonitrile-acide formique 100:0,1 v/v; état MS: source ionique à pulvérisation électrique, détection en mode ionique positif, balayage dans la gamme 150-1200 m/z. On peut utiliser ce procédé pour l'étude pharmacocinétique de la schizadrine B après administration de médicament botanique dissipant la stase sanguine.
Ma Yueming
Shi Rong
Zhang Yongyu
Gowling Lafleur Henderson Llp
Shanghai Sundise Chinese Medicine Technology Development Co. Ltd.
LandOfFree
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