Detection of cellular stress

Details Detection of cellular stress Detection of cellular stress

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) A01K 67/027 (2006.01) C12N 5/10 (2006.01) C12N 15/09 (2006.01)

Patent

CA 2574179

We provide reporter gene constructs incorporating nucleic acid sequences from a promoter region of a gene or set of genes whose expression is modified in response to external or internal changes in the cellular environment such as biochemical stress or toxic conditions. The promoters are operatively linked to nucleic acid sequences chosen on the basis of the ease with which their transcription and/or translation products may be assayed so that the reporter gene constructs can provide a system capable of detecting intracellular conditions. Also described are transfected cell lines including the constructs and transgenic non-human animal models including the constructs.

L'invention porte sur des gènes chimères de reporters comportant les séquences d'acides nucléiques de la région promotrice d'un gène ou d'un ensemble de gènes dont l'expression est modifiée en réponse à des modifications internes ou externes de l'environnement cellulaire, telles que le stress biochimique ou des états de toxicité. Les promoteurs sont fonctionnellement liés à des séquences d'acides nucléiques choisies en fonction de la facilité d'essai de leurs produits de transcription et/ou de traduction, de manière à ce que les gènes chimères reporters puisse constituer un système capable de détecter les états intracellulaires. L'invention porte également sur des lignées de cellules transfectées incluant lesdits gènes chimères et des modèles d'animaux transgéniques incluant lesdits gènes chimères.

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