Detection of chemical mutagens in marker rescue assay

Details Detection of chemical mutagens in marker rescue assay Detection of chemical mutagens in marker rescue assay

C - Chemistry, Metallurgy

12

Q

150/8.5

C12Q 1/68 (2006.01) C12Q 1/02 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 1180649

DETECTION OF CHEMICAL MUTAGENS IN MARKER RESCUE ASSAY ABSTRACT OF THE DISCLOSURE The present application discloses a short-term microbial assay useful in detecting chemical mutagens. A Bacillus subtilis which is infected with a strain of the tem- perate bacteriophage ?105 having a conditional lethal mutation is specifically illustrated. In the assay exogenous DNA from wild-type ?105 is reacted with a potential mutagen and then transfected into the B. subtilis carrying a mutated ?105 DNA. After plating, an increase in the frequency of clear plaques signifies that the test compund is mutagenic. The present invention provides a short-term muta- genicity test which detects frameshift as well as base substi- tution mutations induced by chemicals.

395193

Affiliated with

Also associated with

Rating

Detection of chemical mutagens in marker rescue assay does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Detection of chemical mutagens in marker rescue assay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Detection of chemical mutagens in marker rescue assay will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1216262