Detection of circulating endothelial cells

Details Detection of circulating endothelial cells Detection of circulating endothelial cells

G - Physics

01

N

G01N 33/53 (2006.01)

Patent

CA 2646497

Endothelial cells are detected in a blood sample by enriching the endothelial cells from the blood sample followed by performing on the enriched endothelial cells an immunoassay capable of detecting antigens expressed by the endothelial cells. The immunoassay is capable of detecting antigen expressed from 300 endothelial cells per milliliter of blood. The method can be used for assaying mature circulating endothelial cells or circulating endothelial progenitor cells.

Des cellules endothéliales sont détectées dans un échantillon de sang par enrichissement desdites cellules endothéliales issues de l'échantillon de sang, ces cellules endothéliales enrichies étant soumises à un dosage immunologique capable de détecter des antigènes exprimées par les cellules endothéliales. Le dosage immunologique permet la détection de l'antigène exprimé par 300 cellules endothéliales par millilitre de sang. Cette méthode de dosage peut être utilisée pour des cellules endothéliales en circulation matures ou pour des cellules précurseurs endothéliales en circulation.

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