Detection of conditions or diseases associated with an...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/48 (2006.01) A61K 31/70 (2006.01) A61K 38/00 (2006.01) A61K 38/45 (2006.01) A61K 45/00 (2006.01) A61K 48/00 (2006.01) C12Q 1/68 (2006.01) G01N 33/573 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2245461

Method and compositions are provided for the determination of telomere length and telomerase activity, as well as the ability to inhibit telomerase activity in the treatment of proliferative diseases. Particularly, primers are elongated under conditions which minimize interference from other genomic sequences, so as to obtain accurate determinations of telomeric length or telomerase activity. In addition, compositions are provided for intracellular inhibition of telomerase activity and means are shown for slowing the loss of telomeric repeats in aging cells.

Méthode et compositions servant à déterminer la longueur des télomères et l'activité des télomérases, ainsi qu'à inhiber l'activité des télomérases dans le traitement de maladies à évolution chronique. En particulier, des amorces sont allongées dans des conditions permettant de réduire l'interférence avec d'autres séquences génomiques, afin d'obtenir des déterminations précises de la longueur des télomères ou de l'activité des télomérases. De plus, des compositions sont fournies pour l'inhibition intracellulaire de l'activité des télomérases, et des moyens sont décrits pour ralentir la perte de nouveaux télomères dans les cellules vieillissantes.

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