Detection of delta-bilirubin

Details Detection of delta-bilirubin Detection of delta-bilirubin

C - Chemistry, Metallurgy

12

P

C12P 21/08 (2006.01) C07K 16/18 (2006.01) C07K 16/44 (2006.01) C12N 5/12 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2044227

ABSTRACT A novel method for the determination of authentic delta-bilirubin is provided. This method utilizes a novel monoclonal antibody produced using hybridoma technology and directed specifically against delta-bilirubin rather than against bilirubin that is synthetically or chemically linked to albumin. The antibody is able to distinguish between delta-bilirubin and other forms of bilirubin by recognition of the specific covalent linkage present between bilirubin and albumin in delta-bilirubin. Detection of delta- bilirubin is important in the diagnosis of various hepatobiliary disorders and in the early prognosis of liver transplant patients. As a result, an immunoassay kit for delta-bilirubin detection is also provided.

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