Detection of diarrheogenic shellfish toxins

C - Chemistry – Metallurgy – 12 – P

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C12P 21/08 (2006.01) C07K 16/18 (2006.01) C07K 16/44 (2006.01) C12N 5/18 (2006.01) G01N 33/53 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2093521

An immunoassay of fat-soluble compounds, which comprises conducting an antigen-antibody reaction in the presence of an organic solvent by using an antibody which is resistant to organic solvents. This method enables the antigen-antibody reaction of the fat-soluble compound with the antibody to be conducted by using an extract of a specimen with an organic solvent and serves to simplify the operation. The invention also relates to a monoclonal antibody specific for at least okadaic acid, dinophysistoxin (1) and dinophysistoxin (3); a hybridoma which produces the antibody and a method for immunologically detecting diarrheogenic shellfish poisons by using the antibody. The invention enables all of the three diarrheogenic shellfish poisons to be detected specifically.

L'invention se rapporte à un dosage immunologique de composés solubles dans les graisses, qui consiste à effectuer une réaction antigénique-anticorpal en présence d'un solvant organique en utilisant un anticorps qui résiste aux solvants organiques. Ce procédé permet de procéder à la réaction antigénique-anticorpal du composé soluble dans les graisses avec l'anticorps, en utilisant un extrait d'un spécimen avec un solvant organique, et il sert à simplifier l'opération. L'invention se rapporte également à un anticorps monoclonal spécifique au moins de l'acide okadaïque, de la dinophysistoxine (1) et de la dinophysistoxine (3); à un hybridome qui produit cet anticorps; et à un procédé pour détecter par voie immunologique les poisons de crustacés diarrhéogènes, en utilisant cet anticorps. L'invention permet de détecter spécifiquement chacun de ces trois poisons de crustacés diarrhéogènes.

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