Detection of dna-binding proteins

Details Detection of dna-binding proteins Detection of dna-binding proteins

G - Physics

01

N

G01N 33/53 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2480646

Compositions and methods are provided for detecting and measuring DNA-binding proteins. The compositions and methods permit the simultaneous or near- simultaneous detection of multiple DNA-binding proteins in a multiplex or array format, and can be used to generate profiles of DNA binding activity by proteins, specifically, transcription factors. Multiple protein-DNA binding events in a single sample may be detected and quantitated in a high-throughput format.

L'invention concerne des compositions et des procédés de détection et de mesure de protéines de liaison à l'ADN. Ces compositions et ces procédés permettent la détection simultanée ou quasi simultanée de plusieurs protéines de liaison à l'ADN dans un format multiplex ou un format réseau, et peuvent servir à générer des profils d'activité de liaison à l'ADN par des protéines, plus particulièrement, des facteurs de transcription. Plusieurs événements de liaison protéine-ADN dans un seul échantillon peuvent être détectés et quantifiés dans un format à débit élevé.

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