Detection of dna sequence motifs in ruminants

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2597745

A method for detecting a repeat element in a target ruminant nucleic acid sequence, the method comprising the steps of: (a) contacting a nucleic acid probe capable of hybridizing with a nucleotide sequence flanking said element; and (b) detecting the complex formed between the probe and the target nucleic acid wherein the repeat elements are formed of repeating nucleotide sequences of at least (3) nucleotides.

La présente invention concerne un procédé de détection d'un élément qui se répète dans une séquence d'acides nucléiques ciblée d'un ruminant, ledit procédé comprenant les étapes suivantes : (a) la mise en contact d'une sonde d'acide nucléique capable de s'hybrider avec une séquence nucléotidique flanquant ledit élément ;et (b) la détection du complexe formé par la sonde et l'acide nucléique ciblé dans lequel les éléments répétés sont formés de répétitions de séquences nucléotidiques d'au moins trois (3) nucléotides.

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