Detection of elevated levels of her-2/neu protein from...

G - Physics – 01 – N

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G01N 33/574 (2006.01) A61K 31/4709 (2006.01) A61K 31/517 (2006.01) A61K 39/395 (2006.01) A61K 45/00 (2006.01) A61P 35/00 (2006.01) G01N 33/567 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2684265

The expression of Her-2/neu protein on circulating cancer cells in a sample of blood or peripheral blood mononuclear cells (PBMCs) is detected by performing a sensitive Her-2/neu immunoassay. There is no need to isolate the cancer cells before performing the immunoassay. A positive result indicates the expression of Her-2/neu on cancer cells in the blood sample. This method can be used to identify cancer patients who are likely to benefit from treatment with an anticancer agent that targets Her-2/neu, such as trastuzumab (HERCEPTIN), lapatinib, CP-724,714, NKI-272, and BMS-599626

L'invention concerne l'expression d'une protéine Her-2/neu sur des cellules cancéreuses en circulation dans un échantillon de sang ou des cellules mononucléaires sanguines périphériques (PBMCs) qui est détectée en effectuant un immunodosage sensible à Her-2/neu. Il n'est pas nécessaire d'isoler les cellules cancéreuses avant d'effectuer l'immunodosage. Un résultat positif indique l'expression de Her-2/neu sur des cellules cancéreuses dans l'échantillon de sang. Ce procédé peut être utilisé pour identifier les patients atteints d'un cancer qui sont susceptibles de bénéficier du traitement avec un agent anticancéreux qui cible Her-2/neu, tels que trastuzumab (HERCEPTIN), lapatinib, CP-724,714, NKI-272 et BMS-599626.

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