Detection of elevated levels of her-2/neu protein on...

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G01N 33/574 (2006.01) A61K 39/395 (2006.01) A61P 35/00 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2582713

The expression of Her-2/neu protein on circulating cancer cells in a blood sample is detected by isolating the cancer cells from the blood sample and then performing on the isolated cancer cells a sensitive Her-2/neu immunoassay. A positive result indicates the expression of Her-2/neu on cancer cells in the blood sample. This method can be used to identify cancer patients who are likely to benefit from treatment with an anticancer agent that targets Her-2/neu, such as trastuzumab (HERCEPTIN).

Selon l'invention, l'expression de la protéine Her-2/neu sur des cellules cancéreuses circulantes dans un échantillon sanguin est détectée par isolement des cellules cancéreuses de l'échantillon sanguin et réalisation d'un dosage immunologique Her-2/neu sensible sur les cellules cancéreuses isolées. Un résultat positif indique l'expression de Her-2/neu sur des cellules cancéreuses dans l'échantillon sanguin. Cette méthode peut être utilisée pour identifier des patients atteints d'un cancer pour lesquels un traitement au moyen d'un agent anticancéreux qui cible Her-2/neu, tel que le trastuzumab (HERCEPTINE), pourrait être avantageux.

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