Detection of fungal pathogens

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2313393

Unique DNA sequences are provided which are useful in identifying different pathogenic fungi, such as those which infect grape plants. These unique DNA sequences can be used to provide oligonucleotide primers in PCR based analysis for the identification of fungal pathogens. The DNA sequences of the present invention include the internal transcribed spacer (ITS) of the ribosomal RNA gene regions of particular fungal pathogens, as well as oligonucleotide primers which are derived from these regions which are capable of identifying the particular pathogen.

L'invention concerne des séquences d'ADN uniques utiles pour l'identification de différents pathogènes fongiques (par exemple, du type infectant la vigne). Lesdites séquences peuvent tenir lieu d'amorces oligonucléotidiques dans l'analyse d'amplification en chaîne par polymérase (ACP), pour l'identification des pathogènes fongiques. Ces séquences renferment l'espaceur à transcription interne (ITS) des secteurs de gènes D'ADN ribosomique propres à des pathogènes fongiques spécifiques, ainsi que des amorces oligonucléotidiques issues de ces secteurs et capables d'identifier les pathogènes considérés.

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