Detection of gd2 synthase mrna and uses thereof

Details Detection of gd2 synthase mrna and uses thereof Detection of gd2 synthase mrna and uses thereof

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C12P 19/34 (2006.01) C12Q 1/48 (2006.01)

Patent

CA 2447435

The present invention provides a method to measure GD2 synthase mRNA comprising steps of: (a) obtaining a mRNA sample; (b) performing real-time quantitative RT-PCR on the sample using appropriate primers of GD2 synthase; and (c) determining the amount of GD2 synthase mRNA. The invention also provides a method to diagnose a subject which bears cancer expressing GD2 synthase. Furthermore, this invention provides a method to stage a cancer expressing GD2 synthase in a subject. Finally, this invention provides a kit for detection of GD2 synthase.

Cette invention se rapporte à un procédé servant à mesurer l'ARNm de synthase GD2 et consistant à cet effet: (a) à obtenir un échantillon d'ARNm; (b) à effectuer une réaction RT-PCR quantitative en temps réel sur cet échantillon, en utilisant des amorces appropriées de synthase GD2; et (c) à déterminer la quantité d'ARNm de synthase GD2. Cette invention concerne également un procédé pour diagnostiquer un sujet atteint de cancer exprimant la synthase GD2, ainsi qu'un procédé pour déterminer les stades d'un cancer exprimant la synthase GD2 chez un sujet et, finalement, un kit de détection de synthase GD2.

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