C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2220646
Methods are provided for detecting and quantitating gene sequences, such as mutated genes and oncongenes, in biological fluids. The fluid sample (e.g., plasma, serum, urine, etc.) is obtained, deproteinized and the DNA present in the sample is extracted. The DNA is then amplified using an amplification procedure, such as PCR or LCR, to amplify the mutated gene sequence. In one embodiment, the DNA is contacted with a peptide nucleic acid prior to or during the amplification procedure.
L'invention prote sur des procédés permettant de détecter et de quantifier des séquences de gènes, tels que des gènes et oncogènesayant subi une mutation, dans des liquides biologiques. On prélève l'échantillon liquide (par exemple, plasma, sérum sanguin, urine, etc.) que l'on déprotéinise, puis on extrait l'ADN présent dans l'échantillon. L'ADN est alors amplifié selon une procédure d'amplification, telle que la PCR ou la LCR, afin d'amplifier la séquence de gènes ayant subi une mutation. Dans un mode de réalisation, l'ADN est mis en contact avec un acide nucléique peptidique avant ou pendant la procédure d'amplification.
Riches Mckenzie & Herbert Llp
Trustees Of Dartmouth College
LandOfFree
Detection of gene sequences in biological fluids does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Detection of gene sequences in biological fluids, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Detection of gene sequences in biological fluids will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1520875