Detection of herpes simplex virus types 1 and 2 by nucleic...

Details Detection of herpes simplex virus types 1 and 2 by nucleic... Detection of herpes simplex virus types 1 and 2 by nucleic...

C - Chemistry, Metallurgy

07

H

C07H 21/04 (2006.01) C12N 15/38 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2523016

The present invention relates to a method of detecting the presence or absence of herpes simplex virus (HSV) in a sample based on amplifying a portion of the Glycoprotein G (US4) gene of HSV and detecting the presence of the amplified nucleic acid using primers and detector primers as described herewith. The method of the invention further identifies the type of HSV, either HSV-1 or HSV-2, in a sample. Also encompassed by the invention is a kit comprising the primers and detector primers which may be used with the amplification method described herewith.

La présente invention concerne un procédé de détection de la présence ou de l'absence du virus de l'herpès simplex (HSV) dans un prélèvement. En l'occurrence, on amplifie une partie du gène de la Glycoprotéine G du HSV (US4), et pour détecter la présence d'acide nucléique amplifié, on utilise des amorces et des amorces de détecteur, conformément aux spécifications. Le procédé de l'invention permet également d'identifier dans le prélèvement le type de HSV, soit HSV-1, soit HSV-2. L'invention concerne également un nécessaire comprenant les amorces et des amorces de détecteurs utilisables dans le cadre du procédé d'amplification de l'invention.

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