Detection of hla antigen-containing immune complexes

Details Detection of hla antigen-containing immune complexes Detection of hla antigen-containing immune complexes

G - Physics

01

N

G01N 33/564 (2006.01) G01N 33/569 (2006.01) G01N 33/577 (2006.01) G01N 33/80 (2006.01)

Patent

CA 2138962

The invention provides for methods and compositions to detect the presence of anti-HLA antibodies, HLA antigen or pre- formed HLA-containing immune complexes in biological samples. The complement protein Clq is bound to a solid substrate, then mixed with a biological sample containing immune complexes. The immune complexes are preformed, or formed by adding HLA antigens to a biological sample containing antibodies to HLA, or alternatively, by combining a biological sample contain- ing HLA antigens with defined antibodies to HLA. The immune complexes bind to Clq, and are then detected by the addition of a labeled reagent.

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