Detection of human retrovirus infection

Details Detection of human retrovirus infection Detection of human retrovirus infection

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2204372

A method for determining virus replication in human cells by human retrovirus using RNA amplification comprising detecting the hybridization of an RNA probe which specifically hybridizes with spliced RNA and not with genomic RNA. This method permits early detection of RNA replication resulting from primary infection without detecting non-replicating virus.

Procédé destiné à déterminer une réplication de virus, dans des cellules humaines, par un rétrovirus humain, à l'aide d'une amplification d'ARN, ce procédé consistant à détecter l'hybridation d'une sonde d'ARN, laquelle s'hybride spécifiquement avec l'ARN épissé et non pas avec l'ARN génomique. Ce procédé permet une détection précoce de la réplication de l'ARN consécutive à l'infection primaire, sans détection du virus ne se répliquant pas.

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