C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2200347
Amplification of a target sequence is detected qualitatively or quantitatively by concurrent generation of secondary amplification products labeled with a lipophilic label. The secondary amplification products are designed such that they are generated and cleaved or nicked in a target amplification-dependent manner. This reduces the number of hydrophilic nucleotides linked to the lipophilic label and allows the cleaved or nicked secondary amplification product comprising the lipophilic label to be transferred from the aqueous reaction phase to an organic phase for detection as an indicator of target amplification.
L'invention porte sur un procédé de détection qualitative et quantitative de l'amplification d'une séquence cible par production concurrente de produits secondaires d'amplification marqués à l'aide d'un marqueur lipophile. Les produits secondaires d'amplification sont conçus de manière à pouvoir être produits, coupés, ou cassés en fonction de l'amplification de la cible. Cela réduit le nombre des nucléotides hydrophiles liés au marqueur lipophile et permet le transfert des produits secondaires d'amplification coupés, ou cassés, marqueur lipophile compris, de la phase aqueuse de réaction vers une phase organique en vue de leur détection qui constitue un indicateur de l'amplification de la cible.
Becton Dickinson And Company
Gowling Lafleur Henderson Llp
LandOfFree
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