Detection of individual t-cell reaction patterns against...

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 14/47 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2673432

The present invention relates to a method for identifying the preferential target antigens of anti-tumoral T-cells of a tumor patient, comprising: a) providing T-cells from the blood of at least one tumor patient, b) providing dendritic cells (DCs) and/or B-lymphocytes (BLCs) that are autologous of the tumor patient, wherein the DCs and BLCs have first been transfected with a selection of mRNAs, which code T-cell immunogenic tumor-associated antigens (TAA), and express the same, c) bringing the T-cells in contact with the DCs and/or BLCs, d) identifying those T-cells, which recognize the antigens of the DCs and/or BLCs, and e) identifying the preferential target antigens of anti-tumoral T-cells of the at least one tumor patient based on the T-cells, which recognize the antigens of the DCs and/or BLCs. The method may also comprise the expansion of the T-cells, which recognize antigens of the DCs and/or BLCs. The present invention further relates to a method for the production of an individualized tumor vaccine, or an individualized tumor therapeutic agent, and corresponding methods for the treatment of a tumorous disease utilizing the individualized tumor vaccine, or the individualized tumor therapeutic agent.

L'invention concerne un procédé d'identification des antigènes cibles préférentiels de cellules T anti-tumorales d'un patient atteint d'une tumeur, comprenant : a) la mise à disposition de cellules T provenant du sang d'au moins un patient atteint d'une tumeur ; b) la mise à disposition de cellules dendritiques (CDs) et/ou de lymphocytes B (LCBs) autologues du patient atteint d'une tumeur, sachant que les CDs et les LCBs ont été préalablement transfectés avec une sélection d'ARNm qui codent des antigènes tumoraux (TAA) immunogènes aux cellules T et les expriment ; c) mise en contact des cellules T avec les CDs et/ou les LCBs ; d) identification des cellules T qui reconnaissent les antigènes des CDs et/ou des LCBs ; et e) identification des antigènes cibles préférentiels de cellules T anti-tumorales du patient au moins unique atteint d'une tumeur sur la base des cellules T qui reconnaissent les antigènes des CDs et/ou des LCBs. Le procédé peut en outre comprendre l'expansion des cellules T qui reconnaissent les antigènes des CDs et/ou des LCBs. L'invention concerne en outre un procédé de production d'un vaccin tumoral individualisé ou d'une thérapie tumorale individualisée, ainsi que des procédés correspondants de traitement d'une maladie tumorale en utilisant le vaccin tumoral individualisé ou la thérapie tumorale individualisée.

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