Detection of inducible resistance to...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/18 (2006.01)

Patent

CA 2707033

The embodiments of this invention provide a test panel and method for the detection of inducible resistance to macrolide-lincosamide-streptogramin b(iMLSb) in microorganisms in an automated microorganism identification (ID) and antimicrobial susceptibility determinations (AST) system. The wells of a multiple-well test panel contain a macrolide agent and a lincosamide agent and a combination of both macrolide agent and lincosamide agent. The test panel is inoculated with a broth-suspended microorganism, and placed into the automated microorganism identification (ID) and antimicrobial susceptibility determinations (AST) system. The test panel is incubated within the system and the wells monitored for microorganism growth.

Selon la présente invention, les modes de réalisation concernent un panneau d'essai et un procédé permettant de détecter une résistance inductible aux macrolides, lincosamides et streptogramines de type b (iMLSb) dans des micro-organismes au moyen d'un système d'identification de micro-organismes (ID) et de détermination de la sensibilité aux antimicrobiens (AST) automatisé. Les puits d'un panneau d'essai à puits multiples contiennent un agent macrolide, un agent lincosamide et une combinaison des deux agents précédents. On inocule dans le panneau d'essai un micro-organisme en suspension dans un bouillon de culture. Le panneau est ensuite placé dans le système d'identification de microorganismes (ID) et de détermination de sensibilité aux antimicrobiens (AST) automatisé. On incube le panneau d'essai dans le système et le développement du micro-organisme est suivi dans les puits.

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