Detection of ligand interaction with polymeric material

Details Detection of ligand interaction with polymeric material Detection of ligand interaction with polymeric material

G - Physics

01

N

G01N 3/04 (2006.01) C12M 1/34 (2006.01) C12M 1/40 (2006.01) C12Q 1/25 (2006.01) C12Q 1/68 (2006.01) G01N 33/543 (2006.01) G01N 33/545 (2006.01) G01N 11/16 (2006.01)

Patent

CA 2258239

A method and apparatus for measuring ligand binding to DNA or protein uses changes in the mechanical properties of strips of the DNA or protein to detect the formation of ligand complexes. Samples strips are prepared by pouring protein solution onto a glass surface, drying the solution, and causing the protein to be cross- linked; the cross-linked film is scraped off, cut into strips, and mounted in the stress/strain measuring apparatus, which includes force and strain transducers. The sample is held by arms and can be immersed into solutions. The arms are attached to transducers. The apparatus includes a sample holder. The sample films may include end reinforcements and may be pinned or glued to the arms.

Procédé et appareil permettant de mesurer la liaison d'un ligand à l'ADN ou à une protéine. Ce procédé consiste à utiliser des variations des caractéristiques mécaniques de bandes de l'ADN ou de la protéine pour détecter la formation de complexes à ligands. On prépare des bandes d'échantillons en versant une solution protéique sur une surface de verre, en laissant sécher la solution et en provoquant la réticulation de la protéine; le film réticulé est enlevé par grattage, coupé en bandes et monté dans l'appareil de mesure de contraintes/déformations qui comprend des transducteurs de force et de déformation. L'échantillon est maintenu par des bras et peut être immergé dans des solutions. Les bras sont rattachés aux transducteurs. L'appareil comprend un porte-échantillon. Les films d'échantillon peuvent comprendre des renforcements terminaux et peuvent être épinglés ou collés aux bras.

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