Detection of lymphocyte amplification

Details Detection of lymphocyte amplification Detection of lymphocyte amplification

C - Chemistry, Metallurgy

12

Q

150/8.5

C12Q 1/68 (2006.01) G01N 33/50 (2006.01) G01N 33/569 (2006.01)

Patent

CA 1305912

8003/jg ABSTRACT A method for detecting lymphocyte clonal amplification in a mammal by cloning of lymphocytes so as to identify cell lines expressing a mutation at a structural gene locus and determining the structure of the T-cell receptor in the mutated clonal cell lines. Similar rearrangements of the regions of nucleic acid encoding T-cell receptor among multiple isolated clones from a single individual indicate an in vivo clonal lymphocyte amplification event.

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