Detection of maize fungal pathogens using the polymerase...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01) G01N 1/28 (2006.01)

Patent

CA 2214864

Internal Transcribed Spacer (ITS)DNA sequences from the ribosomal RNA gene region are described for different species and strains of Helminthosporium carbonum, Helminthosporium turcicum, Helminthosporium maydis, Cercospora zeae-maydis, Kabatiella zeae and Puccinia sorghi. Specific primers from within these sequences are identified as being useful for the identification of the fungal isolates using PCR-based techniques.

Description de séquences d'ADN d'espaceur interne transcrit (ITS), provenant de l'ARN ribosomique de différentes espèces et souches d'Helminthosporium carbonum, d'Helminthosporium turcicum, d'Helminthosporium maydis, de Cercospora zeae-maydis, de Kabatiella zeae et de Puccinia sorghi. Les amorces spécifiques provenant de ces séquences permettent de caractériser les isolats fongiques grâce aux techniques fondées sur la PCR.

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