Detection of microorganisms with holographic sensor

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/04 (2006.01) G01N 21/77 (2006.01) G01N 33/483 (2006.01)

Patent

CA 2491889

A method for the detection of a cell comprises immobilising the cell in a device also containing a sensor, and introducing a growth medium, wherein the sensor is sensitive to a product of the cell's growth; and detecting any change in an optical characteristic of the sensor. A device suitable for use in the invention comprises a chamber including a sensor, inlets for a sample and for a growth medium, and means for immobilising an antibody in the chamber or elsewhere in the device that provides a fluidic link with the sensor.

La présente invention concerne un procédé de détection d'une cellule selon lequel : on immobilise la cellule dans un dispositif contenant également un capteur ; on introduit un milieu de croissance, le capteur étant sensible à un produit de la croissance cellulaire ; et on détecte une quelconque modification dans la caractéristique optique du capteur. L'invention se rapporte également à un dispositif utilisé selon le procédé précité, qui comprend une chambre contenant un capteur, des entrées pour un échantillon et pour un milieu de croissance, et des moyens destinés à immobiliser un anticorps dans la chambre ou à un quelconque autre endroit dans le dispositif permettant l'établissement d'une liaison fluidique avec le capteur.

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