Detection of minimal residual disease in lymphoid malignancies

Details Detection of minimal residual disease in lymphoid malignancies Detection of minimal residual disease in lymphoid malignancies

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2303945

The invention relates to the field of cancer diagnosis, more specific to the monitoring of disease development during and after treatment. The invention comprises a method for determining minimal residual disease comprising amplifying nucleic acid molecules using at least one primer reactive with a common gene segment and further comprising identifying malignancy-specific nucleic acid sequences by hybridising with a fluorogenic probe specifically and selectively reactive with said malignancy-specific nucleic acid sequences.

Cette invention porte sur le diagnostic du cancer, et plus spécifiquement sur le contrôle du développement pathologique pendant et après traitement. Cette invention porte donc sur un procédé de détermination d'une pathologie résiduelle minime, ce procédé consistant à amplifier les molécules d'acide nucléique à l'aide d'au moins une amorce réagissant avec un segment génique commun, et à identifier des séquences d'acide nucléique spécifiques de la tumeur maligne en effectuant une hybridation avec une sonde fluorogène réagissant spécifiquement et sélectivement avec des séquences d'acide nucléique spécifiques de la tumeur maligne.

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