Detection of mycobacteria

Details Detection of mycobacteria Detection of mycobacteria

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2192980

The invention provides a method, compositions, and kits useful for detecting mycobacteria in a sample. The method includes contacting the sample with a formaldehyde solution, an organic solvent, and a protein-degrading agent prior to hybridizing a mycobacteria-specific nucleic acid probe to the sample. The invention has particular utility in detection and susceptibility screening of human-disease causing mycobacteria such as Mycobacterium tuberculosis.

L'invention se rapporte à un procédé, à des compositions et à des kits utiles dans la détection de mycobactéries présentes dans un échantillon. Le procédé consiste à mettre l'échantillon en contact l'échantillon avec une solution de formaldéhyde, un solvant organique et un agent de dégradation des protéines avant l'hybridation d'une sonde d'acide nucléique spécifique des mycobactéries sur l'échantillon. L'invention est particulièrement utile dans la détection et la détermination de sensibilité de mycobactéries provoquant des maladies chezl'homme, telles que Mycobacterium tuberculosis.

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