Detection of mycobacteria in clinical material

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2481517

The invention relates to a molecular-biological method for specifically detecting mycobacteria and for differentiating the Mycobacterium tuberculosis complex and Mycobacterium avium from other mycobacteria in clinical material by means of amplification primers and oligonucleotide probes which are specific to the genus-specific and type-specific regions of the 16S-rRNA gene of mycobacteria.

La présente invention concerne un procédé de biologie moléculaire permettant de détecter de manière spécifique les mycobactéries et de différencier la mycobactérie du complexe tuberculosis et la mycobactérie avium d'autres mycobactéries dans un matériau clinique à l'aide d'amorces d'amplification et de sondes oligonucléotidiques qui sont spécifiques aux régions spécifiques au genre et à l'espèce du gène 16S-ARNr de mycobactéries.

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