Detection of negatively charged polymers using...

Details Detection of negatively charged polymers using... Detection of negatively charged polymers using...

C - Chemistry, Metallurgy

07

D

C07D 333/32 (2006.01) C07D 333/20 (2006.01) C07D 409/06 (2006.01) C07D 409/12 (2006.01) C08G 61/12 (2006.01) C12Q 1/68 (2006.01) G01N 21/78 (2006.01) G01N 27/48 (2006.01) G01N 33/53 (2006.01) G01N 33/566 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2442860

Novel methods allowing for the simple optical and electrochemical detection of double-stranded oligonucleotides are disclosed. The methods are rapid, selective and versatile. Advantageously, they do not require any chemical reaction on the probes or on the analytes since they are based on different electrostatic interactions between cationic poly (3-alkoxy-4-methylthiophene) derivatives and single-stranded or double-stranded (hibridized) oligonucleotides.

L'invention concerne des procédés la détection simple, optique et électrochimique d'oligonucléotides double brin. Ces procédés sont rapides, sélectifs et polyvalents. Avantageusement, ils ne nécessitent aucune réaction chimique sur les sondes ou les analytes, du fait qu'ils reposent sur des interactions électrostatiques différentes entre des dérivés poly(3-alcoxy-4-méthylthiophènes) cationiques et des oligonucléotides simple brin ou double brin (hybridés).

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