Detection of no by using guanylyl cyclase and cgmp...

Details Detection of no by using guanylyl cyclase and cgmp... Detection of no by using guanylyl cyclase and cgmp...

C - Chemistry, Metallurgy

12

Q

C12Q 1/26 (2006.01) C12Q 1/42 (2006.01)

Patent

CA 2540822

A method for measuring nitric oxide synthase activity, characterized in the method comprises; (a) providing a solution comprising a nitric oxide synthase and a suitable substrate under conditions which allow synthesis of nitric oxide; (b) adding guanylyl cyclase and GTP under conditions which allow synthesis of cGMP; (c) measuring cGMP.

L'invention concerne une méthode de mesure de l'activité de la synthase d'oxyde nitrique, consistant (a) à utiliser une solution contenant une synthase d'oxyde nitrique et un substrat adapté dans des conditions permettant la synthèse de l'oxyde nitrique; (b) à ajouter une guanylyle cyclase et une GTP dans des conditions permettant la synthèse de cGMP; et (c) à mesurer le cGMP.

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