Detection of nucleic acid hybrids

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2356927

Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of a predetermined endogenous nucleic acid. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, speciation, determination of viral load, genotyping, medical marker diagnostics, and the like.

L'invention concerne des procédés faisant appel à la dépolymérisation d'un hybride d'acide nucléique pour analyser qualitativement et quantitativement la présence d'un acide nucléique endogène prédéterminé. Les applications de ces procédés comprennent la détection de polymorphismes de nucléotides simples, l'identification de changements de bases simples, la spéciation, la détermination de charge virale, le génotypage, les diagnostics de marqueurs médicaux, et les applications correspondantes.

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