Detection of nucleic acid reactions on bead arrays

Details Detection of nucleic acid reactions on bead arrays Detection of nucleic acid reactions on bead arrays

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2370976

The present invention is directed to methods and compositions for the use of microsphere arrays to detect and quantify a number of nucleic acid reactions. The invention finds use in genotyping, i.e. the determination of the sequence of nucleic acids, particularly alterations such as nucleotide substitutions (mismatches) and single nucleotide polymorphisms (SNPs). Similarly, the invention finds use in the detection and quantification of a nucleic acid target using a variety of amplification techniques, including both signal amplification and target amplification. The methods and compositions of the invention can be used in nucleic acid sequencing reactions as well. All applications can include the use of adapter sequences to allow for universal arrays.

La présente invention concerne des techniques et des compositions utilisable sur des microsupports de billes en réseau permettant de détecter et de quantifier un certain nombre de réactions d'acides nucléiques. On trouve des applications de cette invention dans le génotypage, c'est à dire dans la détermination des séquences d'acides nucléiques, plus particulièrement des modifications telles que des substitutions nucléotidiques (mésappariements) et des polymorphismes nucléotidiques simples (SNP). De la même façon, on trouve des applications de cette invention dans la détection et la quantification d'une cible d'acide nucléique utilisant diverses techniques d'amplification, comprenant l'amplification des signaux et de la cible. Les techniques et les compositions de cette invention peuvent aussi être utilisées dans les réactions de séquençage d'acides nucléiques. Toutes les applications peuvent comprendre l'utilisation de séquences d'adaptateur pour permettre l'utilisation de microsupports universels.

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