Detection of nucleic acid sequence modification

Details Detection of nucleic acid sequence modification Detection of nucleic acid sequence modification

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2631586

The invention relates to a non-PCR based method for the detection of a nucleotide modification in a nucleic acid sample comprising contacting a solution comprising nucleic acid sample with a nucleic acid probe in a temperature-controlled and UV illuminated container and measuring the UV absorption of the nucleic acid sample/nucleic acid probe complex.

L'invention concerne un procédé qui n'est pas un procédé à base d'ACP pour la détection d'une modification d'un nucléotide dans un échantillon d'acides nucléiques consistant à mettre en contact une solution comprenant un échantillon d'acides nucléiques avec une sonde à acides nucléiques dans un récipient régulé en température et irradié par des UV et à mesurer l'absorption des UV du complexe échantillon d'acides nucléiques/sonde d'acides nucléiques.

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