C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 19/10 (2006.01) C07H 19/20 (2006.01) C07H 21/00 (2006.01) G01N 27/447 (2006.01)
Patent
CA 2465588
The present invention is directed to a method of detecting pluralities of target nucleic acid sequences by forming and cleaving duplex structures with a pair of probes, one probe of each pair being labeled with an electrophoretic tag. Cleavage of the duplex structures releases electrophoretic tags that are then separated and identified to indicate the presence or quantity of the target sequences. The present invention is particularly useful in multiplex reactions wherein multiple target sequences are detected in one reaction. Kits useful in the detection of nucleic acids are also provided.
L'invention concerne un procédé de détection de pluralités de séquences d'acides nucléiques qui consiste à former et à couper des structures duplex à l'aide de deux sondes marquées d'une étiquette électrophorétique. Le clivage des structures duplex libère les étiquettes électrophorétiques lesquelles sont ensuite séparées et identifiées pour indiquer la présence ou la quantité de séquences cibles. Cette invention est particulièrement appropriée pour les réactions multiplex dans lesquelles les séquences cibles multiples sont détectées en une seule réaction. L'invention concerne en outre des kits utilisés pour détecter les acides nucléiques.
Chenna Ahmed
Singh Sharat
Xiao Vivian
Aclara Biosciences Inc.
Gowling Lafleur Henderson Llp
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