C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
195/1.12, 150/8.
C12Q 1/68 (2006.01) G01N 33/543 (2006.01)
Patent
CA 1313485
DETECTION OF NUCLEIC ACID SEQUENCES USING PARTICLE AGGLUTINATION Abstract of the Disclosure A method of detecting, identifying and/or quantitating nucleic acids in a sample through determination of agglutination or inhibition of agglutination of suspendable particles having nucleic acids bound thereto is described. The nucleic acids can be bound directly to the particle surfaces or attached through a spacer molecule which can, in turn, be either covalently bound or adsorbed to the particle surfaces, The suspendable particles are small enough to remain in suspension and will generally have a large particle size relative to the molecular weight of the DNA or RNA attached to the surfaces. The presence or absence of nucleic acid sequences in a sample is determined by detecting agglutination or inhibition of agglutination of particles having bound thereto nucleic acid se- quences complementary to those of interest in the sample.
530996
Gefter Malcolm L.
Holland Christie A.
Cambridge Biotech Corporation
Swabey Ogilvy Renault
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