Detection of nucleic acids

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) C07H 21/02 (2006.01) C12N 15/11 (2006.01)

Patent

CA 2654016

The present invention relates to compositions and methods for the detection and characterization of small nucleic acid molecules (e.g., RNA (e.g., small RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs)) and other short nucleic acid molecules). More particularly, the present invention relates to methods for the detection and quantification of RNA expression. The present invention further provides for the detection of miRNA and siRNA variants.

La présente invention concerne des compositions et des procédés destinés à la détection et à la caractérisation de petites molécules d'acide nucléique (par exemple, l'ARN (notamment les petits ARN tels que les micro ARN (ARNmi) et les petits ARN interférants (ARNsi)) et d'autres molécules de petits acides nucléiques). Plus particulièrement, la présente invention concerne des procédés destinés à la détection et à la quantification de l'expression de l'ARN. La présente invention concerne en outre la détection de variants d'ARNmi et d'ARNsi.

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