Detection of nucleic acids by formation of...

Details Detection of nucleic acids by formation of... Detection of nucleic acids by formation of...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2223774

A method is disclosed for detecting a target polynucleotide sequence. The method comprises incubating an oligonucleotide with the target polynucleotide sequence and a nucleotide polymerase under isothermal conditions wherein at least one nucleotide is added to the 3'-terminus of the oligonucleotide to provide an extended oligonucleotide having the additional nucleotides. The presence of extended oligonucleotide is detected as an indication of the presence of the target polynucleotide sequence. The method has particular application to the detection of DNA.

L'invention a pour objet un procédé de détection d'une séquence polynucléotidique cible. Ce procédé consiste à incuber un oligonucléotide avec la séquence polynucléotidique cible et une nucléotide polymérase dans des conditions isothermiques, selon lesquelles au moins un nucléotide est ajouté à l'extrémité 3' de l'oligonucléotide pour produire un oligonucléotide étendu, comportant les nucléotides supplémentaires. La présence de l'oligonucléotide étendu est détectée comme un signe de la présence de la séquence polynucléotidique cible. Le procédé s'applique particulièrement à la détection d'ADN.

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