C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) C12N 9/12 (2006.01) C12N 9/22 (2006.01) C12P 19/34 (2006.01)
Patent
CA 2284552
The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure- specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of nucleic acid from various viruses in a sample.
L'invention porte sur des moyens de détection et de caractérisation de séquences d'acide nucléique et des variations de ces mêmes séquences. L'invention porte également sur des procédés de formation de structures de clivage d'acide nucléique sur une séquence cible et de clivage de ladite structure de clivage d'une manière spécifique d'un site. L'activité de nucléase, spécifique à la structure, d'une variété d'enzymes sert à cliver la structure de clivage dépendant de la cible, indiquant par là la présence de séquences spécifiques d'acide nucléique ou de leurs variations spécifiques. L'invention porte en outre sur des procédés de détection de produits de clivage non cibles recourant à la formation d'une région de fixation de protéines complète et activée, et sur des procédés sensibles et spécifiques permettant de détecter les acides nucléiques parmi les différents virus d'un échantillon.
Brow Mary Ann D.
Hall Jeff G.
Kwiatkowski Robert W.
Lyamichev Victor I.
Mast Andrea L.
Smart & Biggar
Third Wave Technologies Inc.
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