Detection of nucleic acids by target-specific hybrid capture...

Details Detection of nucleic acids by target-specific hybrid capture... Detection of nucleic acids by target-specific hybrid capture...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2629978

Target-specific hybrid capture (TSHC) provides a nucleic acid detection method that is not only rapid and sensitive, but is also highly specific and capable of discriminating highly homologous nucleic acid sequences. The method produces DNA:RNA hybrids which can be detected by a variety of methods.

La présente invention concerne une capture d~hybrides spécifique à la cible (TSHC) représentant un procédé de détection d~acide nucléique qui est non seulement rapide et sensible, mais également extrêmement spécifique et capable de différencier des séquences hautement homologues d~acides nucléiques. Le procédé produit des hybrides d'ADN : ARN pouvant être détectés par divers procédés.

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