C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01) C12Q 1/70 (2006.01)
Patent
CA 2204641
Thermophilic Strand Displacement Amplification (tSDA) for amplification of nucleic acid target sequences in situ in cells in suspension, on slides or in tissues is described. Excellent specimen morphology is preserved, and either DNA targets, RNA targets, or both may be selectively amplified. In situ amplification by tSDA is compatible with immunochemical techniques, so that both amplification of target sequences and immunological staining can be performed on the same specimen.
Cette invention se rapporte à l'amplification par déplacement de brins thermophiles (tSDA) qui permet l'amplification de séquences cibles d'acides nucléiques in situ dans des cellules en suspension, sur des lamelles ou dans des tissus. On préserve l'excellente structure morphologique d'échantillons, et il est possible d'amplifier sélectivement soit des cibles d'ADN, soit des cibles d'ARN, soit ces deux types de cibles. L'amplification in situ par tSDA est compatible avec les techniques immunochimiques, de telle sorte qu'il est possible d'effectuer à la fois l'amplification des séquences cibles et la coloration immunologique sur le même échantillon.
Lohman Kenton L.
Ostrerova Natalie V.
Reid Robert Alan
Van Cleve Mark
Becton Dickinson And Company
Gowling Lafleur Henderson Llp
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