Detection of plant pathogenic fungi

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2206031

Root rots are serious conifer nursery diseases. At present, seedlings are inspected visually to determine whether they show symptoms of disease. Cylindrocarpon destructans and Cylindrocladium floridanum are two important causal agents of root rots. A substantially more efficient and reliable method of detecting the presence of these fungi directly from infected tissue involves the use of a set of DNA primers which can be used in conjunction with the polymerase chain reaction (PCR) to create species specific probes for detecting species of each fungi.

Le pourridié est une grave maladie des pépinières de conifères. Actuellement, les semis sont inspectés visuellement pour déceler les symptômes de la maladie. Cylindrocarpon destructans et Cylindrocladium floridanum sont deux importants agents étiologiques du pourridié. Une méthode nettement plus efficace et plus sûre pour déceler la présence de ces champignons directement à partir de tissus infectés fait appel à un ensemble d'amorces d'ADN, qui peuvent être utilisées conjointement à la réaction en chaîne de la polymérase pour produire des sondes spécifiques détectant individuellement les espèces de champignons.

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